Composite
Ptac-TEV-P

Part:BBa_K4225012:Design

Designed by: Leon Ritchie Salim, Janviere Yau   Group: iGEM22_HKUST   (2022-10-09)


Ptac-TEV-Pc-RFP-CS-LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1020
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 855
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1701
    Illegal AgeI site found at 1813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 439
    Illegal SapI.rc site found at 787


Design Notes

Source

All parts except TEV are taken from biobricks. Refer to the basic part BBa_K4225009 for the source of TEV. This composite part is also codon optimized for our chassis, DH5α.

References